“EAS Journal of Biotechnology and Genetics” ISSN 2663-189X (Print) and ISSN 2663-7286(Online) is monthly, peer reviewed, open access Academic and Research Journal which publishes Full Length Original Research Articles, Review Articles, Mini Reviews, Essays, Short Communications, Case Studies, editorial comments etc in all the fields of Biotechnology and Genetics.
Scope of Journal
The aim of the “EAS Journal of Biotechnology and Genetics” is to promote excellence by providing a venue for academicians, Researchers and students to publish significant empirical and conceptual findings in all the fields of Biotechnology and Genetics and their applied fields and share globally.
“EAS Journal of Biotechnology and Genetics” publishes in all the fields of Biotechnology and Genetics like Biophysics, Biochemistry, Bioinformatics, Bioenergy, Biosafety, Biosecurity, Bioethics, Medical Biotechnology, Pharmaceutical biotechnology, agricultural biotechnology, Molecular genetics, Evoluationary genetics, Developmental genetics, Heredity genetics, Behavioural genetics, Genetic analysis, Gene regulation, Gene expression profiling, Genetic variation, Epigenetics, Gene therapies, Cellular genetics and molecular biology, Population genetics, Quantitative and computational genetics, Microbial genetics, Genetics in medical field, Signal transduction, Genome and systems biology, genetic disorders, Computational molecular biology, rDNA, CRISPR, and all other genetic and molecular biology techniques etc.
Editor in Chief:
Dr. T. Selvankumar
Professor & Head PG & Research Dept. of Biotechnology (DST-FIST Sponsored) Mahendra Arts & Science College (Autonomous) Kalippatti, Namakkal- 637 501, Tamil Nadu, India Email: firstname.lastname@example.org
Mary Esien Kooffreh
Dept of Genetics and Biotechnology, University of Calabar, P.M.B 1115 Calabar, Nigeria Email: email@example.com
Dr. Benard Chemwei, PhD
Researcher and Academic Kabarak University Private Bag 20157, Kenya Email: firstname.lastname@example.org
Dr Nouar Hind
Department of Ecology and Plant Biology, Faculty of Nature and Life Sciences, Ferhat ABBAS University, Setif -1, 19000, Algeria Email: email@example.com
Ms.c, Anatomy and Cell Biology, Shahid Sadoughi University of Medical Science and Health Services, Yazd, Iran. Email: firstname.lastname@example.org
Asma Mami Maazoun
Post-doctoral researcher, Laboratory of Biotechnology Applied to Agriculture (LBAA); National Agricultural Research Institute of Tunisia, Avenue rue Mokhtar Attia 70, 1000 Tunis, Tunisia Tel: (00216) 29883165 E-mail: email@example.com
Nduche, Michael Ugochukwu
Department of Plant Science and Biotechnology, Michael Okpara University of Agriculture Umudike Abia State, Nigeria Email: firstname.lastname@example.org
Ordu University, Department of Molecular Biology and Genetics, Faculty of Science and Letters, 52200 Ordu-Turkey Email: email@example.com
Jiménez Leiva Alejandra, Madrigal Elizondo José Antonio, Murillo Muñoz Melissa, Rojas Lezama Marilyn, Villegas Campos Mauricio, Mora Román Juan José
East African Scholars J Biotechnol Genet, 2020; 2(1):1-11.
DOI : 10.36349/EASJBG.2020.v02i01.001
Cancer is a disease that has positioned itself as the second cause of death globally. However, there is a deficiency in diagnostic tools and treatment. Traditionally, biopsy involves tissues extraction to be examined. But, it is an invasive procedure and can lead to complications in patients. Liquid biopsy has been developed as a non-invasive technique, with lower cost and allowing a better diagnosis. As a complement, new-generation sequencing (NGS) allows information sequencing and analysis from DNA. The combined use of these tools can result in identification of genetic aberrations in cancer, improving of patients´ prognosis, and use of personalized therapies when studying an individual´s genomics, epigenomics, and proteomics profile. Despite these advances, there are elements to improve procedures sensitivity, a better understanding of biochemistry and immunological response in tumorigenesis processes, and integration of this data through computational strategies for the development of tumor profiles
This study was initiated to generate information about genetic variability, heritability and genetic advance of
yield components in Citrullus mucosospermus. In this way, four inbred lines of C. mucosospermus were evaluated at
Kononfla city in Western Côte d’Ivoire, using a randomized complete block design with three repetitions. The results of
discriminant analysis indicated significant differences between four C. mucosospermus inbred lines what are Bebu,
Wlêwlê small seeds 1, Wlêwlê small seeds 2 and Wlêwlê small seeds 3. Mahalanobis distances varied significantly from
8.44 to 629.50 between theses inbred lines. All investigated traits recorded high genotypic and phenotypic coefficients of
variation values except fruit maturity period and fruit diameter where, low values were recorded. Studied morphological
traits exhibited high heritability (H²> 80%) coupled with moderate to high genetic advance as percentage of mean.
Stepwise regression analysis revealed mass of fresh seeds per fruit followed by mass of fruit, seed width, seed length and
fruit maturity as traits contributing for linear increase in mass of dry seeds. These traits could be considered as key
criteria for selecting high yielding line in C. mucosospermus breeding program at Côte d’Ivoire.
The experimental application of induced spawning on grass carp (Ctenopharyngodon idella) were carried out
during the period of spawning extended from April to May 2016. The different doses level of human chorionic
gonadotropin (HCG) and carp pituitary homogenate (CPH) were used separately or mixed together. The present results
indicated that the best dose level that give higher number of fertilized and hatching eggs were 5mg/kg of body weight of
CPH, following 9hours with 1500IU HCG per kg of body weight. No significance differences occurred (P> 0.05) in
fertilized and hatching rates between HCG and CPH of treatment groups. In all experiment, the initial egg diameter
before the initial injection was 600 micron in diameter. In mature stage, the ripe eggs were recorded to be more than 1
mm in diameters after injection with hormonal treatment.
The sex steroid hormones includes17β-estradiol (E2), testosterone (T) and progesterone (P) were studied
during the annual cycle of gonadal maturation. The pituitary gland secretes gonadotropin hormone which is used as
monitor and control on the secretion steroid hormones from the theca and granulose cells of follicular oocyte. The annual
fluctuated in 17β-estradiol (E2) is correlated with the degree of maturation state in the ovary of grass carp as
vitellogenesis ceased and the ova reached to the final maturation state, the concentration of E2 decreased to the minimum
level 109ng/ml. The testosterone (T) level among the other sex steroid hormones plays an essential role in the sexual
maturation. Testosterone (T) fluctuated through the maturation cycle and reached the lowest value at immature state of
gonad and measured 1.5ng/ml, however this value gradually increased and peaked in ripe stage to 2.2ng/ml. The value of
T decreased to 1.5ng/ml in atretic stage. The fluctuated in the total T level in the serum of grass carp seem to be
inconsistent with fluctuation of E2. Progesterone (P) peaked in maturing ovary and measured 12.9 ng/ml and then
decreased to 9.2 in ripe stage. Both Follicle stimulating hormone (FSH) and luteinizing hormone (LH) play an important
role during the annual cycle for growth oocyte and is necessary for maturation and successful in propagation process.
Medicinal plants have been used as remedies for human diseases because they contain components of therapeutic value. Plants serve as the greatest source to obtain new antimicrobials. An increasing interest in herbal remedies has been observed in several parts of the world. Tridax procumbens Linn. commonly known as ‘coat button’ is a weed and a pest plant. Previous researches have shown that the plant has antimicrobial activities and pharmacological effects. This study aimed at evaluating the phytochemical compounds and antibacterial activities of the leaf extract of Tridax procumbens. Leaf ethanol and water extracts were used in this study. The test organisms were Staphylococcus aureus and Escherichia coli. The antibacterial assay was performed by disc diffusion method. Treatment extract concentrations were 250mg/ml, 200mg/ml, 100mg/ml and 50mg/ml and distilled water (negative control) and an antibiotic Amphicloxa (positive control). Each treatment was replicated three times. The leaf extract was tested for the presence of tannins, saponins, flavonoids, terpenoids, alkaloids and glycosides. The plates were examined for zones of inhibitions. Data collected was subjected to analysis of variance. Treatments were separated and compared using LSD at P < 0.05. Both ethanol and water extracts were biologically active against the two microbes. Ethanol extract showed greater inhibition (9.29 mm) in comparison to water extract (7.19 mm). Escherichia coli showed greater susceptibility as compared to Staphycoccus aureus. The phytochemical screening of the leaf extract of Tridax procumbens revealed the presence of tannins, alkaloids, saponins, flavonoids, terpenoids and glycosides. It is possible that the growth inhibition observed in the study occurred due to the presence of these different chemical compounds. From these findings, ethanolic and water extracts of the leaf of Tridax procumbens Linn. showed a good potential as a source of new drug for treating infections caused by
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