Malnutrition is one of the major public health problem around the under developed country and particularly in Côte d'Ivoire. The nutritional status is one of the best world indicator of the individual well-being. The aim of this study was to evaluate the effect of Spirulina supplementation on certain serum biochemical parameters in growing wistar rat (Rattus norvegicus) for three months. Four homogeneous groups of six rats each, contained three males and three females, were used. Group 1 (control) received conventional food (FACI® granules) ad libithum and distilled water at a rate of 10 ml/kg of body weight while groups 2, 3 and 4, received FACI® granules, Spirulina at 10 (Group 2), 50 (Group 3) and 100 mg/kg (Group 4) of body weight. Blood samples collected from the orbital sinus permitted to take 5 ml of blood that were centrifuged at 3000 rpm for 10 min. The serum collected was used to determine biochemical parameters. Analysis of results revealed that transaminases (AST and ALT) decreased significantly (P < 0.05) at 50 and 100 mg/kg bw. Lipids measured out as well as glycemia knew also a significant (P < 0.05) decrease at all doses. As for creatinine and urea, they have all known a significant (P < 0.05) decrease as well as total and conjugated bilirubins. Total protein increased significantly (P < 0.05) at 50 and 100 mg/kg b.w. In conclusion, Spirulina supplementation acts favorably on blood biochemical parameters and could therefore contribute to improve certain vital organs functioning, in particular liver and kidneys.