Introduction: Pregnancy can be a source of anxiety stressors, especially in primigravida mothers' third trimester of pregnancy. One of the efforts made by health workers to reduce the level of anxiety in pregnant women facing the delivery process is by providing health services and counseling, and health education during pregnancy check-ups (ANC). Every woman believes that pregnancy is a natural thing that must be lived. On the other hand, some argue that it is an event that determines the next life. Anxiety and anxiety in pregnant women, if not treated seriously, will impact and affect the physical and psychological of both the mother and the fetus. Objective: This study aims to analyze the relationship between knowledge and the level of anxiety of primigravida TM III in dealing with childbirth at the Wae Rii Health Center and Langke Rembong Regency. Methods: The research design used in this research is descriptive correlative with a cross-sectional approach. A sample of 70 respondents was selected using the purposive sampling technique using inclusion and exclusion criteria. Data on pregnant women were taken from the register book and distributed in knowledge and anxiety questionnaires. Statistical test using chi-square test. Results: Most of the 35 people with good knowledge had a mild level of anxiety. The results of the statistical test showed a p-value of 0.000 which means a p-value <0.05, so there is a statistically significant relationship between knowledge and the anxiety level of TM III primigravida mothers in dealing with labor at the Work Center of Manggarai Regency, especially Wae Rii and Langke Rembong District.