Background: Stress is state of mental or emotional strain or tension resulting from adverse or demanding circumstances. Novice nurses perception of job stress is a global concern and work-related stress has the potential to negatively affect the individual’s psychological and physical health, as well as an organization’s effectiveness. Objectives: The aim of the present study was to examine thelevel of novice nurses perception of job stress working at hospital. Method: Simple convenience technique was used for this study and sample size was 108. The study was carried out within the period of March 25, 2017 to April 7, 2018. Data was collected by using self-structured questionnaire. The method of data collection was self-admitted questionnaire. Data was analyzed by both descriptive and inferential statistics by using SPSS version 23. Result: Results revealed that the most stressful event was the uncertainty concerning the treatment, workload, death and dying, followed by problems with supervisors, conflict with physicians, problems with peers, inadequate emotional preparation, patients and their families and discrimination. Nurses working in medicine ward and emergency department had high level of stress job stress compared with than nurses who were working in the other wards. Conclusion: The study concludes that nurse managers in hospitals should develop programs to reduce job stress of novice nurses through in-service education program or and also conduct more systematic survey to better understand the work stress of not only novice nurses related to clinical practice job stress but also other nurses.