Background: Oral health problems affected 55.6% of adolescents aged 10-14 in Indonesia. This can be prevented by maintaining oral hygiene. Many physical and psychological changes happen in the adolescent phase, and more attention is needed to maintain oral hygiene. Oral hygiene is influenced by various factors, including demographic and socioeconomic factors. Objectives: This study aimed to determine factors associated with oral hygiene among adolescents in Jember, Indonesia. Method: There were 409 students from eight different junior secondary schools in Jember Regency as participants in this study. The sample was obtained using the cluster random sampling method. Data on oral hygiene as a dependent variable was collected through the OHI-S examination. Data on sex, school, ethnicity, mother and father education, father and mother employment, monthly income, and number of children as independent variables was gathered from questionnaires and students' data from the school database. First, a univariate Spearmen test was run for all independent variables (p<0.05). The significant variables were then analyzed using multiple regression to determine factors associated with oral hygiene (p<0.05). Results: The average OHI-S score was 2.53, which was categorized as moderate. Multivariate analysis results showed that females were more likely to have better OHIS-S scores than males (B=-0.716; p=0.000); ethnic Javanese were more likely to have better OHI-S scores than Maduranese (B=0.293; p=0.004); and middle-income students more likely have better OHI-S than low-income students (B=-0.307; p=0.003). Conclusion: It can be concluded from this study that adolescents' socioeconomic background is associated with their oral hygiene. This suggests the need to give more attention to a new model of oral health promotion programs targeting gender, ethnicity, and lower-income groups.