H. pylori is a micro-aerophilic, gram-negative, S-curved, polar motility, spiral bacillus measuring 2.5 µm to 5 µm long and 0.3 µm wide. Type two diabetes of mellitus is a metabolic disorder characterized by chronic hyperglycemia associated with disturbances of the various metabolisms, in particular lipid, carbohydrate and protein. In this study, we aimed to determine the prevalence of H. pylori infection in the diabetic and non-diabetic population consulting in the gastroenterology and external medicine department of CHU-Brazzaville. We carried out a descriptive cross-sectional study over a period from June 20 to November 02, 2021, like a period of six months. Ninety patients were selected divided into two groups, each subdivided into two subgroups. 44 type two diabetes of mellitus patients and 46 non-diabetic patients were included. The average age of diabetic patients was 51 ± 11 years old with an average duration of diabetes evolution of 5.54 ± 4.58 years old. The average age of non-diabetic patients was 40±15 years old. Out of 90 patients in our study population, we had a predominance of women (68) compared to men (22). The overall frequency of H. pylori infection of the study population was 57%. It was 66% in diabetic patients and 48% in non-diabetics. The results of our study showed that H. pylori infection was more common in diabetics compared to non-diabetics.