EAS Publisher | Appraisal of a New Histological Staining Method of Helicobacter pylori with Rubia cordifolia

Appraisal of a New Histological Staining Method of Helicobacter pylori with Rubia cordifolia

DOI : https://doi.org/10.36349/easjbg.2025.v07i02.003

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Background: Among different histological staining methods for Helicobacter pylori, the Haematoxylin and Eosin (H&E) staining method is commonly used in most clinical laboratories. Rubia cordifolia, a herbaceous climber, commonly known as the Madder plant is well known for its colour which is commercially exploited in the textile industry. In this study, we explored whether this colouring agent can be utilized for staining H. pylori in histological sections. Methods: At first, we studied the nature and intensity of the Rubia root extract with different solvents and mordants. Then we observed that an excellent orange-yellow colour was developed when acetic acid was mixed with ethanol extract of the root powder. Results: A combined Haematoxylin and this extract not only stained H. pylori in an excellent way, but also revealed hidden bacteria in gastric pits and mucus very easily. Conclusion: A combined haemaxylin and Rubia (H&R) stain appear ideal for the demonstration of H. pylori in histological sections.

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