Background: Female Genital Mutilation/Cutting (FGM/C) remains a major public health issue in Isiolo County, Kenya, despite national declines. It is linked to serious obstetric and neonatal complications. However, nurses and midwives often lack the necessary knowledge and skills to manage such cases. Targeted training interventions can strengthen their capacity to improve preventive practices and clinical care. Methods: A quasi-experimental study with pre- and post-intervention assessments was conducted among nurses and midwives in selected hospitals in Isiolo County. Data were collected using structured questionnaires to assess knowledge, attitudes, sociocultural influences, and preventive practices related to FGM/C-associated obstetric and neonatal complications. The intervention comprised structured training sessions focused on clinical management, cultural competence, and legal frameworks. Data were analyzed using descriptive statistics, paired samples t-tests, and chi-square tests to determine the significance of changes pre- and post-intervention. Results: The study revealed significant improvements in knowledge and preventive practices among nurses and midwives following the training intervention. Knowledge scores increased significantly (p < 0.05) across domains related to immediate physical, gynecological, obstetric, and sexual harms of FGM/C. Attitudes toward FGM/C-related complications shifted positively, with increased recognition of the professional responsibility to intervene. Post-intervention, a higher proportion of respondents reported confidence in identifying and managing FGM/C complications and advocating against the practice. Despite these gains, some sociocultural barriers persisted, highlighting the complexity of changing attitudes in culturally entrenched settings. Conclusion: Targeted training interventions significantly enhance nurses’ and midwives’ knowledge, attitudes, and preventive practices concerning FGM/C-related obstetric and neonata