Background: Red blood cell (RBC) aggregability and deformability are determining factor of blood viscosity, microcirculatory flow, and oxygenation of tissues. These hemorheological properties can be modified by different pharmacological agents such as anesthetics. This study explored the impact of two widely used anesthetic drugs, ketamine and lidocaine, on aggregability and deformability of the RBCs in male Wistar rats. Methodology: The male Wistar rats (N= 35) were subdivided into five groups of six each. Group 1 was a control which was subjected to distilled water. Group 2 got 2 mg/kg lidocaine (plain), Group 3 got 2mg/kg lidocaine with adrenaline, Group 4 experienced 5mg/kg ketamine and Group 5 had a combination of 5mg/kg ketamine with 2mg/kg lidocaine. The treatments were done on two days. At the termination of treatment rats were sacrificed and blood samples were taken through cardiac puncture. The deformability of the RBCs was determined through diffraction ektacytometry and automated rheoscopy, and the aggregability indices were determined through rheoscopes based on light transmission at rest. Analysis of data was done through ANOVA where the significance was p < 0.05. Results and Discussion: The results indicated that there were no significant changes in RBC aggregability and deformability of all treatment groups against the controls. The ketamine alone or the combination of ketamine and lidocaine had no effect on the erythrocyte rheological behaviour. These findings are in line with the past studies that have shown that these anesthetics have insignificant effects on blood viscosity, hematocrit or plasma rheology. Conclusion: Ketamine and lidocaine do not negatively impact on RBC aggregability and deformability of male Wistar rats. Their application seems to be hemorheologically secure with no threat of causing the blood viscosity and microcirculatory flow disruption.