Aim: This study was conducted to evaluate the knowledge levels of nurses working in children’s hospitals in Al-Hilla, Iraq, regarding pain management in children and to examine certain variables associated with knowledge levels. Method: This descriptive cross-sectional study was carried out with nurses working in pediatric units of three children’s hospitals in Al-Hilla. The study sample consisted of 255 nurses. Data were collected using the Introductory Information Form and the Nurses’ Knowledge Form on Pain Management in Children. Descriptive statistics and the chi-square test were used for data analysis. Results: The overall level of knowledge regarding pain management among nurses was found to be moderate. The lowest level of knowledge was identified in the subdimension related to nursing care for children experiencing pain. Statistically significant relationships were found between knowledge scores and educational level as well as the unit in which the nurses worked. Nurses working in pediatric emergency units, neonatal units, and pediatric intensive care units had higher knowledge scores. Conclusion: The results of the study indicate a need to improve the knowledge levels of nurses working in pediatric units regarding pain management. Strengthening structured educational programs and standard practice guidelines, particularly those focusing on nursing care and pain assessment, is recommended.