Inflammatory Bowel Disease refers to a collective term that encompasses various chronic, non-specific disorders of the gastrointestinal tract, including Ulcerative Colitis (UC) and Crohn's Disease (CD). UC is limited to the rectum and colon, impacting both the mucosa and submucosa. The current study aimed to explore the in-vivo intestinal anti-inflammatory properties of a polyherbal plant extract, which is a blend of the roots of Hemidesmus indicus and the seeds of Centratherum anthelminticum. Inflammation was effectively induced through intra-rectal administration of 4% glacial acetic acid in albino Wistar rats via the intra-rectal route to evaluate the acute intestinal anti-inflammatory effects. The positive control group received only the vehicle, while other groups were administered oral treatments with two different doses (500 mg/kg and 1000 mg/kg body weight) along with Prednisolone (2 mg/kg body weight). The animals treated with the polyherbal plant extracts exhibited a significant reduction in colon scores compared to the positive control group. There was a notable decrease in Myeloperoxidase and Lipid Peroxidation levels when compared to the positive control group. The treatment with polyherbal plant extracts is likely responsible for the significant intestinal anti-inflammatory effects observed, potentially through an antioxidant mechanism.