Water pollution is a worldwide issue caused by human interference with nature. The physicochemical variables of water samples of the New Calabar River, Choba section were studied to develop guideline data on the pollution status. Water samples were obtained from three stations based on effluent characteristics. The physicochemical characteristics were analysed using standard conventional techniques. The findings showed the difference concentrations of selected physicochemical parameters with the use of one- way ANOVA to analyze the results in three locations. During the study, the physicochemical parameters of surface water in wet and dry seasons were measured using standard methods for temperature, total dissolved solids, turbidity, electrical conductivity, pH, salinity, dissolved oxygen, chemical oxygen demand, and nitrate. The results showed that temperature varied between 27.90a ± 0.97 and 29.02 ± 1.71a ℃ across the stations and in both seasons with a mean TDS of 172.52 ±2.43 which is significantly different across stations and between the seasons. Turbidity recorded the highest value of 22.98a ± 12.66 NTU in station 1. All the values recorded in the three station in both dry and wet season were above the permissible limit. Higher and lower conductivity value of 474.03±5.37a and 279.17±5.92c µS/CM was recorded in station 1 and 3 during the dry season. pH of surface water varied from 7.08± 0.27b to 8.53±0.22a Mg/l in the two seasons while a higher salinity was recorded during the dry season across the stations. DO ranged from 5.53c ± 0.88 to 6.65±0.24a mg/l across the stations and in both seasons. Others were COD that ranged from 120.02±2.36c to 223.02±5.67a mg/kg in both seasons. Turbidity and nitrate were above the WHO and NESREA permissible limit. There was a seasonal variation between the dry and wet season in most of the parameters studied. This study has shown that despite the visibility anthropogenic activities in the study area, the surface water is still