ABSTRACT
Introduction: Acute myelomonocytic leukemia with an eosinophilic component (AML4Eo) is a particular and rare hematological entity, representing between 5% and 8% of all acute myeloid leukemias. The aim of our work is to highlight the clinical and cytogenetic epidemiological particularities of this pathology at the Hassan II University Hospital in FES. Patients and Methods: Retrospective descriptive study spread over 14 years (January 2009-January 2023) including all patients diagnosed with LAM4Eo.Myelogram reading as well as immunophenotyping by flow cytometry on Cytomic FC500 were performed at the central hematology laboratory and cytogenetics at the genetics laboratory CHU HASSAN II of FES. Clinical data were collected from patients' medical charts. Results: Thirteen patients were enrolled. The mean age at diagnosis was 20.4 years, with a predominance of males (54%) most patients presented with an altered general condition, signs of cytopenia and a tumor syndrome. All patients presented with profound anemia associated with thrombocytopenia and hyperleukocytosis. Blood smears showed the presence of peripheral blasts in 80% of cases. All medullograms met the morphological criteria established by the FAB (Franco-American-British) classification for the diagnosis of LAM4Eo.Cytochemical staining with myeloperoxidase was performed on all medullograms and was positive in all cases. Immunophenotyping was carried out in 56% of patients, showing the existence of very immature blasts expressing myeloblast and monoblast markers. Four of our patients underwent cytogenetic studies, with no translocation or inversion of chromosome 16. Three patients died immediately after diagnosis. The other patients were put on chemotherapy, two of whom died during treatment. Discussion/Conclusion: LAM4Eo is a rare hematological malignancy with a better prognosis than other myeloid leukemias. Despite the development of cytogenetics and molecular biology techniques, morphological examination ..

ABSTRACT
Background: Obesity has been recognized as an important risk factor for hyperuricemia although the exact mechanism is not well understood. The goal of this study is to evaluate serum uric acid levels in obese subjects. Methods: A total of 100 individuals with 50 obese patients and 50 healthy non-obese controls. Serum uric acid, total cholesterol (TC), low density lipoprotein cholesterol (LDL - C), high density lipoprotein cholesterol (HDL - C) and triglycerides (TGs) were measured. Serum uric acid was evaluated using uricase method. The serum TC was estimated using modified Liebermann – Burchard reaction. HDL – C estimation involved a two – staged procedure using Liebermann Burchard reaction, LDL – C was calculated using Friedwald’s equation while TGs were estimated using enzymatic methods on commercial kits. Results: In this study, serum uric acid levels were significantly higher in the obese group compared to healthy controls (510.0 + 72.5 Vs 320.0 + 34.2: p<0.001). Conclusion: Hyperuricemia has been observed in patients with obesity and therefore the need to screen all obese patients for serum uric acid levels.

ABSTRACT
Vitamin D is a crucial hormone in phosphocalcium homeostasis. Although vitamin D intoxication is rare, its complications can be dramatic, affecting both the short-term vital prognosis and long-term renal function. We report here a case of hypercalcemia due to vitamin D intoxication, with the aim of analyzing the factors that contribute to vitamin D intoxication, assessing the consequences of this intoxication on the child's health, and proposing recommendations for appropriate management. The patient was a 6-year-old child with a history of common nutritional rickets for which he had been receiving vitamin D supplementation. The patient was admitted to the pediatric emergency department of Children's Hospital in Rabat for incoercible vomiting and acute dehydration. Biological assessment showed severe hypercalcemia at 150mg/l, hypercalciuria at 192 mg /24 hours. The serum level of 25(OH) vitamin D was greater than 154 ng/ml. The diagnosis retained was as hypercalcemia due to vitamin D intoxication, confirmed by an in-depth interview with the parents, who found a over administration of the recommended dose. The evolution was marked by chronic kidney disease due to nephrocalcinosis (creatinine clearance according to the SCHWARTZ formula 17 ml/min). Heterogeneity of the expression forms of vitamin D dosages and intake regimens leads to confusion and increases the risk of misuse, as in the case of our patient, where a misreading of the medical prescription led to the intentional ingestion of toxic doses, hence the need for close and adequate medical supervision and awareness of vitamin D supplement use among healthcare professionals and parents.