ABSTRACT
Since the world wide spreading of SARS-COV2 2,406,745 suspected cases with 165,257 death ones have been recorded. COVID-19 is a highly contagious disease that spread via direct exposure to the droplets generated when an infected person cough or sneeze or touching surfaces that are contaminated with those droplets and then toughing the nose, eyes, mouth or any mucous membrane. The deleterious impact of COVID-19 on all life sectors and without the availability of specific treatment or protective vaccine, researchers started the adoption of repurposing principle to help with the management and containment of the virus. As we are running out of time, the discovery and development of new selective anti-COVID19 drugs is not a practical option; leaving us with the enrollment of already available drugs in this crisis. Many drugs are currently under clinical trials suggesting to decrease the mortality of infected patients using the theory of "cytokine storm", hyper inflammatory status as an example have shown that “oxidant storm” instead of cytokine storm is the primary pathogenesis cascade for causing mortality in the late stages of COVID-19. In this review article, we repurpose the well-known antibiotic Rolitetracycline as adjunctive management of COVID-19 suspected or infected patients based on the documented non antibiotic activities of tetracycline and hiring the docking technique.

ABSTRACT
The present study was to evaluate the anti-anemic power of total aqueous extract of Moringa oleifera leaves (TAEMo) on phenylhydrazine induced anemic Wistar rats. First, hemolytic anemia was induced in rats by intraperitoneal administration of phenylhydrazine (PHZ) at the dose of 40 mg/kg body weight for two consecutive days. Then, the anemic rats were orally treated daily for 26 days with aqueous extract of Moringa oleifera leaves (TAEMo) at doses of 400, 800 and 1600 mg/kg b w. and Ranferon®-12(Reference substance). The rats were analyzed for erythrocyte parameters such as erythrocyte cells, hemoglobin, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration on day 0, 2, 5, 14 and 28. The results revealed that the aqueous extract restored rapidly the modified erythrocyte parameters at the end of treatment. TAEMo at 1600 mg/kg showed an effect similar to the reference substance, Ranferon®-12. The study of reversibility after stopping treatment showed that not all erythrocyte parameters studied were significantly affected. This study found anti-anemic properties of Moringa oleifera leaves aqueous extract thus confirming the use of this plant in the treatment of anemia in the population.

ABSTRACT
Background: Wound infection is a manifestation of disturbed host–bacteria equilibrium in a traumatized tissue environment in favor of the bacteria. A wound infection not only has the possibility to elicit a systemic response (sepsis), but is highly likely to inhibit the multiple processes. Materials and Methods: Solid lipid nanoparticles (SLNs) were prepared using glycerol tristearate and poloxamer by solvent evaporation method. The particle size, polydispersity index (PDI), zeta potential (ZP), FTIR, NMR, water vapor transmission rate determination and in vivo wound healing studies were performed. Results: The average particle size results showed that the nanoparticle size of N2 was in the range of 0.4-0.8 µm and polydispersivity index less than 1. The prepared hydrogels had WVTR in the range of 2187.9 g/m2 to 2954 g/m2 could maintain a suitable moist environment in the wound that could enhance the wound contraction and tissue regeneration, thereby accelerating wound healing. Heliotropium indicum extract SLN Hydrogel significantly contributed to wound healing compared with the blank control group at 15 and 18 day post-wounding (P < 0.05). Conclusion: Nanoparticle containing Heliotropium indicum extract could be released slowly from Carboxymethyl chitosan oxidized alginate hydrogel hydrogel in a sustained manner to stimulate fibroblast proliferation, capillary formation and collagen production that can significantly effect on the healing phases. As a result, we suggested that the prepared hydrogel might have great potential application in the wound healing.

ABSTRACT
Background: One of the leading causes of morbidity and mortality amongst type 2 diabetic (T2DM) patients is Cardiovascular disease (CVD). Patients with T2DM are at a higher risk of developing CVD than non-diabetic patients. Statin therapy has been proven to be effective for the primary prevention of CVD amongst T2DM patients. The objective of this study was to determine the extent of statin therapy use for primary prevention of CVD in T2DM patients at TTH. Methods: A retrospective review of prescription records of T2DM patients who attended the Diabetes Clinic between January 1 2019 to March 31 2019 was conducted. Patient-specific data, evidence of T2DM Diagnosis and prescribed statin therapy were retrieved. Data was collected using a pre-structured tool and analyzed with STATA 15.0. Results: A total of 171 patients were included in this study. Of these, only 49%(84) patients received a statin. Atorvastatin 20mg was prescribed for 76%(64) patients, followed by atorvastatin 10mg for 22%(19) patients. Atorvastatin 40mg and simvastatin 20mg was the least prescribed at 1%(1) patient each. A total of 76% of statin therapies were of moderate-intensity dose, 23% were of low-intensity dose and only 1% was of high-intensity dose. Conclusion: Despite the overwhelming evidence supporting the cardiovascular benefits of statins in T2DM patients, these therapeutic agents are under-prescribed for T2DM patients at TTH with atorvastatin being the most frequently prescribed statin therapy.

ABSTRACT
Propoxazepam, 7-bromo-5-(o-chlorophenyl)-3-propoxy-1,2-dihydro-3H-1,4-benzodiazepin-2-one, in the models of nociceptive and neuropathic pain showed significant analgesic activity and also has an anticonvulsant effect, which explains the analgesic component of the pharmacological spectrum. Mechanism of propoxazepam anticonvulsant properties includes GABAergic and glycinergic systems. Antibradykinin and antileukotriene action, dopaminergic system, NMDA, and alpha-1 adrenergic receptors, except the prostaglandin component, are involved in the mechanisms of propoxazepam analgesic effect. Previous pharmacokinetic studies in mice have shown that [214C]Propoxazepam evacuation from stomach (intragastral administration, 10 mg/kg) described as two-phase process (first phase with kel -0,68 h-1, the second of kel = 0,0094 h-1). Total dose quantity, absorbed during experiment, was ~ 80%, with absorption constant of 0,371 ± 0,098 h-1. Similar values of distribution volume (743 ± 195 sm3/kg and 1090 ± 421 g/kg for blood and brain respectively) let suggest intensive mass transfer. LD50 of propoxazepam is greater than 5000 mg/kg and it therefore, belongs to the category V of relatively non-toxic substances according to the GHS. In the acute toxicity study, neither mortality no significant change in the body weight and the relative organ weights were recorded in all treated mice and rats. Propoxazepam did not show the ability to induce gene mutations in the microplate version of the test (Muta-ChromoPlate kit) on the strains Salmonella typhimurium TA 98 and TA 100. The metabolic activation system was also not effective, that is, Propoxazepam is neither a "direct" nor an "indirect" mutagen for Ames strains. The present investigation demonstrates the analgetic and anticonvulsant effects, good bioavailability and safety of propoxazepam suggesting its promising potential for pharmaceutical uses.

ABSTRACT
Objective: The main objective of present study was to develop and validate a reverse phase chiral high performance liquid chromatographic method for R & S enantiomeric separation of Lercanidipine hydrochloride in API as well as tablet dosage form. Methods: The R-Isomer of Lercanidipine Hydrochloride was resolved on a Chiral OJ-H (150 x 4.0mm, 5um ) column using mobile phase system containing 10mM Ammonium acetate and Acetonitrile in the ratio of (35:65 v/v). The mobile phase was set flow rate of 1.0ml and the volume injected was 10ul for every injection. The detection wavelength was set at 240nm. Results: The proposed method was productively applied for the quantitative determination of (R)-Isomer in Lercanidipine hydrochloride in API as well as tablet dosage form. The linear regression analysis data shows good linear relationship over a concentration range 0.5ug/ml to 4ug/ml for (R)-Isomer. The mean value of the correlation coefficient was 0.998 for (R)-Isomer. The method was validated as per the ICH guidelines. The detection limit (LOD) was about 0.05ug/ml and quantisation limit (LOQ) was about 1.0ug/ml for (R)-Isomer. The relative standard deviation was found to be 2.0% for (R)-Isomer of Lercanidipine Hydrochloride. Conclusion: The method is specific, rapid, precise and accurate for the separation and determination of (R)-isomer in (S) - Lercanidipine hydrochloride in API & tablet dosage form. The developed and validated HPLC method and the statistical analysis showed that the method is repeatable and selective for when compared to earlier published research articles for the estimation of (R)-Isomer of the Lercanidipine Hydrochloride drugs substances as well as in tablet dosage form.