ABSTRACT
This study was initiated to generate information about genetic variability, heritability and genetic advance of yield components in Citrullus mucosospermus. In this way, four inbred lines of C. mucosospermus were evaluated at Kononfla city in Western Côte d’Ivoire, using a randomized complete block design with three repetitions. The results of discriminant analysis indicated significant differences between four C. mucosospermus inbred lines what are Bebu, Wlêwlê small seeds 1, Wlêwlê small seeds 2 and Wlêwlê small seeds 3. Mahalanobis distances varied significantly from 8.44 to 629.50 between theses inbred lines. All investigated traits recorded high genotypic and phenotypic coefficients of variation values except fruit maturity period and fruit diameter where, low values were recorded. Studied morphological traits exhibited high heritability (H²> 80%) coupled with moderate to high genetic advance as percentage of mean. Stepwise regression analysis revealed mass of fresh seeds per fruit followed by mass of fruit, seed width, seed length and fruit maturity as traits contributing for linear increase in mass of dry seeds. These traits could be considered as key criteria for selecting high yielding line in C. mucosospermus breeding program at Côte d’Ivoire.

ABSTRACT
The experimental application of induced spawning on grass carp (Ctenopharyngodon idella) were carried out during the period of spawning extended from April to May 2016. The different doses level of human chorionic gonadotropin (HCG) and carp pituitary homogenate (CPH) were used separately or mixed together. The present results indicated that the best dose level that give higher number of fertilized and hatching eggs were 5mg/kg of body weight of CPH, following 9hours with 1500IU HCG per kg of body weight. No significance differences occurred (P> 0.05) in fertilized and hatching rates between HCG and CPH of treatment groups. In all experiment, the initial egg diameter before the initial injection was 600 micron in diameter. In mature stage, the ripe eggs were recorded to be more than 1 mm in diameters after injection with hormonal treatment.

ABSTRACT
The sex steroid hormones includes17β-estradiol (E2), testosterone (T) and progesterone (P) were studied during the annual cycle of gonadal maturation. The pituitary gland secretes gonadotropin hormone which is used as monitor and control on the secretion steroid hormones from the theca and granulose cells of follicular oocyte. The annual fluctuated in 17β-estradiol (E2) is correlated with the degree of maturation state in the ovary of grass carp as vitellogenesis ceased and the ova reached to the final maturation state, the concentration of E2 decreased to the minimum level 109ng/ml. The testosterone (T) level among the other sex steroid hormones plays an essential role in the sexual maturation. Testosterone (T) fluctuated through the maturation cycle and reached the lowest value at immature state of gonad and measured 1.5ng/ml, however this value gradually increased and peaked in ripe stage to 2.2ng/ml. The value of T decreased to 1.5ng/ml in atretic stage. The fluctuated in the total T level in the serum of grass carp seem to be inconsistent with fluctuation of E2. Progesterone (P) peaked in maturing ovary and measured 12.9 ng/ml and then decreased to 9.2 in ripe stage. Both Follicle stimulating hormone (FSH) and luteinizing hormone (LH) play an important role during the annual cycle for growth oocyte and is necessary for maturation and successful in propagation process.

ABSTRACT
Medicinal plants have been used as remedies for human diseases because they contain components of therapeutic value. Plants serve as the greatest source to obtain new antimicrobials. An increasing interest in herbal remedies has been observed in several parts of the world. Tridax procumbens Linn. commonly known as ‘coat button’ is a weed and a pest plant. Previous researches have shown that the plant has antimicrobial activities and pharmacological effects. This study aimed at evaluating the phytochemical compounds and antibacterial activities of the leaf extract of Tridax procumbens. Leaf ethanol and water extracts were used in this study. The test organisms were Staphylococcus aureus and Escherichia coli. The antibacterial assay was performed by disc diffusion method. Treatment extract concentrations were 250mg/ml, 200mg/ml, 100mg/ml and 50mg/ml and distilled water (negative control) and an antibiotic Amphicloxa (positive control). Each treatment was replicated three times. The leaf extract was tested for the presence of tannins, saponins, flavonoids, terpenoids, alkaloids and glycosides. The plates were examined for zones of inhibitions. Data collected was subjected to analysis of variance. Treatments were separated and compared using LSD at P < 0.05. Both ethanol and water extracts were biologically active against the two microbes. Ethanol extract showed greater inhibition (9.29 mm) in comparison to water extract (7.19 mm). Escherichia coli showed greater susceptibility as compared to Staphycoccus aureus. The phytochemical screening of the leaf extract of Tridax procumbens revealed the presence of tannins, alkaloids, saponins, flavonoids, terpenoids and glycosides. It is possible that the growth inhibition observed in the study occurred due to the presence of these different chemical compounds. From these findings, ethanolic and water extracts of the leaf of Tridax procumbens Linn. showed a good potential as a source of new drug for treating infections caused by

ABSTRACT
Medicinal plants have been used as remedies for human diseases because they contain components of therapeutic value. Plants serve as the greatest source to obtain new antimicrobials. An increasing interest in herbal remedies has been observed in several parts of the world. Tridax procumbens Linn. commonly known as ‘coat button’ is a weed and a pest plant. Previous researches have shown that the plant has antimicrobial activities and pharmacological effects. This study aimed at evaluating the phytochemical compounds and antibacterial activities of the leaf extract of Tridax procumbens. Leaf ethanol and water extracts were used in this study. The test organisms were Staphylococcus aureus and Escherichia coli. The antibacterial assay was performed by disc diffusion method. Treatment extract concentrations were 250mg/ml, 200mg/ml, 100mg/ml and 50mg/ml and distilled water (negative control) and an antibiotic Amphicloxa (positive control). Each treatment was replicated three times. The leaf extract was tested for the presence of tannins, saponins, flavonoids, terpenoids, alkaloids and glycosides. The plates were examined for zones of inhibitions. Data collected was subjected to analysis of variance. Treatments were separated and compared using LSD at P < 0.05. Both ethanol and water extracts were biologically active against the two microbes. Ethanol extract showed greater inhibition (9.29 mm) in comparison to water extract (7.19 mm). Escherichia coli showed greater susceptibility as compared to Staphycoccus aureus. The phytochemical screening of the leaf extract of Tridax procumbens revealed the presence of tannins, alkaloids, saponins, flavonoids, terpenoids and glycosides. It is possible that the growth inhibition observed in the study occurred due to the presence of these different chemical compounds. From these findings, ethanolic and water extracts of the leaf of Tridax procumbens Linn. showed a good potential as a source of new drug for treating infecti